| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ong, Thea (10)
|
MAUNSJ |
|
38.45 |
- |
| 2 |
Kiesel, Addison E (10)
|
MASJAC |
|
39.89 |
- |
| 3 |
Cosello, Nina E (10)
|
MAJW |
35.33 |
42.40 |
7.07 |
| 4 |
Baron, Isabella M (10)
|
MAECCC |
47.76 |
45.20 |
-2.56 |
| 5 |
Jack, Olivia M (9)
|
MAJW |
43.30 |
46.20 |
2.90 |
| 6 |
Jarantow, Alexandra K (9)
|
MAPENN |
|
47.87 |
- |
| 7 |
Montgomery, Talyah N (10)
|
MASJAC |
|
49.41 |
- |
| 8 |
Merch, Laura A (10)
|
MASJAC |
52.96 |
51.62 |
-1.34 |
| 9 |
Bridgeford, Grace B (9)
|
MAECCC |
|
53.33 |
- |
| 10 |
Corrielus, Jayna A (10)
|
MASJAC |
|
54.74 |
- |
| 11 |
Johnson, Olivia M (9)
|
MAUNUD |
|
55.17 |
- |
| 12 |
Jacob, Emily C (9)
|
MAWAVE |
|
55.21 |
- |
| 13 |
Ethiraj, Sanjita (9)
|
MAT85 |
|
57.63 |
- |
| 14 |
Cottrell, Lia J (9)
|
MASJAC |
|
58.27 |
- |
| 15 |
Davis, Adelaide Q (9)
|
MAECCC |
1:13.10 |
1:21.44 |
8.34 |
|
Huntsinger, Haley M (10)
|
MAUN |
34.10 |
NS |
- |
|
Leinenbach, Lea M (9)
|
MAYBCC |
|
NS |
- |